empty negative control plasmid (pcdna3.1 Search Results


an empty control pcdna 3.1/+ plasmid (pc/c)  (Ribobio co)
90
Ribobio co an empty control pcdna 3.1/+ plasmid (pc/c)
Overexpressing CITED2 also restored cancer cell proliferation and chemoresistance in gastric cancer cells with FGD5-AS1 downregulation. (A) In sh-FGD5AS1-transduced SGC-7901 and MKN-28 cells, a mammalian expression plasmid containing the whole cDNA sequence of human CITED2 (pc/CITED2), or an empty control pcDNA <t>3.1/+</t> plasmid <t>(pc/C),</t> was transfected into cells. After 48 h, qRT-PCR was carried out to verify the transfection efficiency (* P < 0.05). (B) A 5-day MTT assay was carried out to compare the proliferation rates between SGC-7901 and MKN-28 cells infected with sh-FFG5AS1 and pc/C, and those infected with sh-FFG5AS1 and pc/CITED2 (** P < 0.05). (C) In double-infected SGC-7901 and MKN-28 cells, 5-FU chemoresistance was compared between cells infected with sh-FFG5AS1 and pc/C, and those infected with sh-FFG5AS1 and pc/CITED2 (** P < 0.05).
An Empty Control Pcdna 3.1/+ Plasmid (Pc/C), supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
an empty control pcdna 3.1/+ plasmid (pc/c) - by Bioz Stars, 2026-03
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Overexpressing CITED2 also restored cancer cell proliferation and chemoresistance in gastric cancer cells with FGD5-AS1 downregulation. (A) In sh-FGD5AS1-transduced SGC-7901 and MKN-28 cells, a mammalian expression plasmid containing the whole cDNA sequence of human CITED2 (pc/CITED2), or an empty control pcDNA 3.1/+ plasmid (pc/C), was transfected into cells. After 48 h, qRT-PCR was carried out to verify the transfection efficiency (* P < 0.05). (B) A 5-day MTT assay was carried out to compare the proliferation rates between SGC-7901 and MKN-28 cells infected with sh-FFG5AS1 and pc/C, and those infected with sh-FFG5AS1 and pc/CITED2 (** P < 0.05). (C) In double-infected SGC-7901 and MKN-28 cells, 5-FU chemoresistance was compared between cells infected with sh-FFG5AS1 and pc/C, and those infected with sh-FFG5AS1 and pc/CITED2 (** P < 0.05).

Journal: Frontiers in Genetics

Article Title: Long Non-coding RNA FGD5-AS1 Regulates Cancer Cell Proliferation and Chemoresistance in Gastric Cancer Through miR-153-3p/CITED2 Axis

doi: 10.3389/fgene.2020.00715

Figure Lengend Snippet: Overexpressing CITED2 also restored cancer cell proliferation and chemoresistance in gastric cancer cells with FGD5-AS1 downregulation. (A) In sh-FGD5AS1-transduced SGC-7901 and MKN-28 cells, a mammalian expression plasmid containing the whole cDNA sequence of human CITED2 (pc/CITED2), or an empty control pcDNA 3.1/+ plasmid (pc/C), was transfected into cells. After 48 h, qRT-PCR was carried out to verify the transfection efficiency (* P < 0.05). (B) A 5-day MTT assay was carried out to compare the proliferation rates between SGC-7901 and MKN-28 cells infected with sh-FFG5AS1 and pc/C, and those infected with sh-FFG5AS1 and pc/CITED2 (** P < 0.05). (C) In double-infected SGC-7901 and MKN-28 cells, 5-FU chemoresistance was compared between cells infected with sh-FFG5AS1 and pc/C, and those infected with sh-FFG5AS1 and pc/CITED2 (** P < 0.05).

Article Snippet: A mammalian expression plasmid (pcDNA 3.1/+) containing the whole cDNA sequence of human CITED2 (pc/CITED2) and an empty control pcDNA 3.1/+ plasmid (pc/C) were purchased from RiboBio (RiboBio, Guangzhou, China).

Techniques: Expressing, Plasmid Preparation, Sequencing, Transfection, Quantitative RT-PCR, MTT Assay, Infection